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Top/fop flash reporter assay

WebI have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and FOP, I couldn't see a high TOP/FOP ratio. WebLuciferase reporter assays were performed by transfecting TOP/FOP Flash (A), Hes1 (B) or Hey1 (C) luciferase reporter in the cells treated with or without rapamycin (Rapa, 200nM, Sigma), an ...

Dual functions of TET1 in germ layer lineage bifurcation …

Web17. júl 2024 · TOP/FOP-flash reporter assays. Cells were seeded in a 96-well plate, and the TOP/FOP-Flash (Genechem) was co-transfected into cells along with LINC01278 siRNA or overexpression plasmid. Luciferase activity was measured using the Promega Dual-Luciferase Reporter Assay System at 24 h after transfection. The luciferase activity was … Web1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点 … tanaz bruna https://astcc.net

LncRNA CASC11 promotes the cervical cancer ... - Biological …

Web9. apr 2024 · Download Citation Mechanism of VPS26 gene promoting implant osseointegration through Wnt/β-catenin pathway in hyperlipidemia rats Objective: To investigate the mechanism of VPS26 effect on ... Web因此，将数个拷贝的TCF/LEF的 DNA结合位点(AGATCAAAGGgggta，大写碱基为DNA结合序列，小写的碱基为间隔序列)克隆至含TA病毒最小启动子(Minimal TA viral promoter)的萤火虫荧光素酶报告系统载体中，构建得到TOP-Flash质粒(Millipore,MA)。本研究将TOP-Flash质粒与脂质体2000(Invitrogen ... WebTOPflash (TCF Reporter Plasmid) 21-170 Sigma-Aldrich TOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase mi More>> tanazapine

TCF Reporter Plasmid Kit - Sigma-Aldrich

Category:Top Flash Fop Flash Dual Luciferase Reporter Assay System Bioz …

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Top/fop flash reporter assay

Does anyone have experience with the TOPFLASH assay?

Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay was used to analyze the activity of Wnt/β-catenin signaling pathway. The vector pRL-SV40 was served as the internal reference. Cells in the control groups were co-transfected with pRL-SV40 and TOP/FOP flash (Promega). Cells in NC groups were co-transfected with pRL-SV40, TOP/FOP flash and si … WebThe Luciferase Reporter 1000 Assay System (Cat.# E4550) was designed to meet the needs of users who perform a large number of assays, particularly in 96-well plates. The system contains sufficient reagents to perform 1,000 luciferase assays (100µl per assay).

Top/fop flash reporter assay

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WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0 ... Web11. apr 2024 · 1.5 × 10 4 SW620 or Difi cells were plated in 24-well plates, then co-transfected with the firefly luciferase reporter plasmid TOP-FLASH or FOP-FLASH and the Renilla luciferase vector phRG-TK at 1 µg/ml (kindly provided by Dr P. Blache, IRCM, Montpellier, France) and incubated with oxaliplatin (5 µg/ml). 72 h post-transfection, cells …

Web22. mar 2024 · The ratio of TOP/FOP indicated the activity of Wnt/β-catenin signaling pathway. Luciferase reporter assay Fragments of the β-catenin promoter (predicted from position −2000 bp to +44 bp) was cloned into the pGL3-Basic Vector (Promega) to generate β-catenin promoter reporter plasmids. Web22. sep 2024 · The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously. It has broad applications across various fields of cell and molecular biology—wherever you want to …

Web86. Millipore luciferase top flash. Luciferase Top Flash, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF binding sites that were cloned into the pGL3 vector (Promega), which contains a minimal promoter followed by a firefly luciferase open reading frame.

WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. …

Web• Established the use of the TOP/FOP FLASH Luciferase Assay in Dr. Wei’s lab. • Used TOP/FOP FLASH Luciferase Assay to monitor levels of canonical Wnt-activated transcription as a proxy for ... batam 3d2n itineraryWebPočet riadkov: 10 · TOPflash (TCF Reporter Plasmid) 21-170 Sigma-Aldrich TOPflash (TCF … batam 3d2n packageWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : … tanazaWeb31. máj 2024 · In vitro proliferation, migration and invasion assays, and an in vivo nude mice model were utilized to assess the important role of desmosomal genes on SOX30-induced tumor suppression. A WB assay and TOP/FOP flash reporter assay was used to investigate the downstream pathway regulated by the SOX30-desmosomal gene axis. tanaza travelWeb1. mar 2024 · Furthermore, the TOP/FOP-Flash reporter assay revealed that Wnt/β-catenin pathway was significantly inactivated in DAX1-silenced cervical cancer cells with the downregulation of Wnt/β-catenin... bata m400Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a … tanaz gouldWeb背景技术微小RNA（miRNA）在肿瘤的发生和发展中起着至关重要的作用。以前，我们表明miR-504被下调并抑制胶质母细胞瘤（GBM）中的肿瘤增殖。然而，miR-504在GBM间充质（ME）过渡中的调控及其相关机制仍不清楚。方法转录组和临床数据来自癌症基因组图谱（TCGA）数据库。 tanazacq sylvain justice