The human caco-2 iec cell line

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WebJul 27, 2024 · These molecules are biosynthesized by gut microbes and secreted into the intestinal lumen, where they modulate the activities of many host cell types, including intestinal epithelial cells (IECs), and, for metabolites that are able to penetrate the gut epithelium, immune cells within the lamina propria. WebJun 1, 1993 · The effect of exogenous nucleotides on the proliferation and differentiation of enterocytes was comparatively studied using a human colon tumor cell line (Caco-2) and a normal rat small intestinal crypt cell line (IEC-6). Caco-2 cells exhibited more active endogenous nucleic acid metabolism than did IEC-6 cells, as evidenced by greater cellular ...

Caco-2 Cell Line - an overview ScienceDirect Topics

WebCytokine production has been studied in human and rat epithelial cell lines such as Caco-2, HT-29, and IEC-6.18 22-24 T84, HT-29, and SW620 cell lines secreted substantial amounts of IL-8 after stimulation with IL-1β, TNF-α or interferon … WebAbstract The Src homology-2 domain-containing tyrosine phosphatase 2 (SHP-2) regulates many cellular processes, including proliferation, differentiation and survival. Polymorphisms in the gene enco... christian christmas poems for cards

Non-pathogenic bacteria elicit a differential cytokine response by ...

WebThe epithelial cells of the gastrointestinal tract are the first to encounter ingested nucleotides. Enterocytes metabolize or transport nucleotides (often partially metabolized) … WebJan 19, 2024 · Human colorectal tumour-derived cell lines, such as Caco-2, T84, and HT-29, as well as rodent-derived cell lines, such as IEC-6 and IEC-18, provide replicable and high-throughput models of the intestinal epithelium [20,21], whilst organ-on-a-chip cultures have begun to replicate more in vivo-like epithelial properties and behaviours . These ... WebThe Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of … george the animal steele height

Colon Carcinoma Cell Line - an overview ScienceDirect Topics

Human Intestinal Epithelial Cell Line Caco2 ATCC Bioz

WebFigure 2: ECACC CACO-2 cells (ECACC catalogue no. 09042001) showing uniform tight junction formation. Heterogenous cell populations can be observed, which is typical of … WebA study of a human colon carcinoma cell line (Caco-2) showed that cell proliferation was altered by systematic variation in extracellular EhCySS over a range (0 to −150mV) that occurs in human plasma (Jonas et al., 2002). From: Methods in Enzymology, 2010 Related terms: In Vitro Neoplasm Programmed Cell Death Metastatic Carcinoma Tumor … george the bear hoffmeisterWeb20.6.2.1 Intestinal permeability models. Caco-2 cell lines are the principle cell type for studies of nanoparticle translocation. Caco-2 cells are not used as individual cells but … george the butcher paterson nj

"WebDec 20, 2024 · In the present study, an in vitro approach is used to evaluate the anti-inflammatory properties of osthole, and its potential use as a drug. In current experiments, … " - The human caco-2 iec cell line

The human caco-2 iec cell line

The human intestinal epithelial cell line Caco-2; …

WebFigure Legend Snippet: Comparison of degrees of adherence to Caco 2 cells evaluated in a semi-quantitative scale (0–no adherence, 1–weak adherence, 2–moderate adherence, ... WebThe Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of …

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WebTransport of these compounds across the filter grown IEC-18 cells was compared with transport across the human colon carcinoma Caco-2 cells. Results: in IEC-18 cells, … WebApr 10, 2024 · Inflammation and oxidative damage in the human colon adenocarcinoma cell line (Caco-2) were induced by dextran sulfate sodium/hydrogen peroxide. In addition, spleen CD4+ T lymphocytes of rats were sorted in vitro. ... Ephrin-B reverse signaling induces expression of wound healing associated genes in IEC-6 intestinal epithelial cells. World J ...

WebThe human colorectal adenocarcinoma-derived Caco-2 cell line was evaluated as a means isolating common respiratory viruses from nasopharyngeal aspirates for the diagnosis of respiratory diseases. One hundred eighty-nine direct immunofluorescence positive nasopharyngeal aspirates obtained from patients with various viral respiratory diseases … WebHuman Iec Line Caco 2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol …

WebCaco-2 cell lines exhibit morphological structures similar to human small intestinal epithelial cells. [16] [17][18] Therefore, we adopted this strain as a representative of cultured colon ... WebCaco-2 on the apical chamber were exposed to LPS and/or a mixture of palmitic and oleic acid (PAOA) for 24 h. FITC-4000 dextrans (FD4) permeability across Caco-2 monolayer was increased by the treatment of Caco-2 cells with PAOA and LPS, consistently with tight junction-associated proteins reduction.

WebNov 10, 1997 · The Caco-2 cell line originating from a human colorectal carcinoma, and IEC-18 cells originating from rat ileum epithelium were obtained from the American Type …

WebCells form tight junctions within 5 days of thawing and can be used on Days 5–8 post-thaw Cells express intestinal markers (e.g., villin and CDX2) at high levels Drug metabolizing … george the baker cincinnatiWebCaco-2 [Caco2] are epithelial cells isolated from colon tissue derived from a 72-year-old, White, male with colorectal adenocarcinoma. This cell line is a suitable transfection host and has applications in cancer and toxicology research. Product category Human cells Organism Homo sapiens, human Cell type epithelial cell Morphology Epithelial-like george the barber covent gardenWebMar 7, 2024 · Objective: We investigated whether co-incubation of 5-FU and gum-based cerium oxide nanoparticles (CeO 2 NPs) would improve half-maximal inhibitory concentration (IC 50) and apoptosis in the Caco-2 cancer cell line Materials and Methods: In this experimental study, we synthesized Ceo-2-XG by the nano perception method.X-ray … george the cat video christian christmas phone wallpaperWebCell culture. The Caco-2/15 cell line, a stable clone of the parent Caco-2 cells (42), has been characterized elsewhere (8, 50). Caco-2/15 cells are known to be poor secretors of growth factors (2, 8, 12, 17). Cells between passages 53 and 70 were cultured in plastic dishes (60 or 100 mm; Falcon Plastics, Los george the chipmunk tik tokWebCaco-2 (from Cancer coli, "colon cancer") is an immortalized cell line of human colorectal adenocarcinoma cells. It is primarily used as a model of the intestinal epithelial barrier. [1] … george the chimney guy - wayneWebTransport of these compounds across the filter grown IEC-18 cells was compared with transport across the human colon carcinoma Caco-2 cells. Results: in IEC-18 cells, transepithelial transport of GlySar and methylglucose was as fast as the transport of mannitol, which is transported passively via the paracellular route. christian christmas powerpoint template