Phospho ire1 antibody
WebHowever, accumulation of unfolded proteins results in dissociation of BIP from IRE1 and oligomerization of IRE1 accompanied by the upregulation of GRP78/BIP which is consistent with our results [53]. Activation of IRE1 initiates the recruitment of the TRAF2/ASK1 complex and activate the Jun-N-terminal kinase (JNK), one of the stress kinases [54]. WebIRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa.
Phospho ire1 antibody
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WebProduct name Anti-IRE1 (phospho S724) antibody [EPR23107-16] See all IRE1 primary antibodies Description Rabbit monoclonal [EPR23107-16] to IRE1 (phospho S724) Host species Rabbit Tested applications Suitable … WebMar 9, 2024 · IRE1α Antibody (B-12) is a mouse monoclonal IgG 1 κ IRE1α antibody, cited in 50 publications, provided at 200 µg/ml raised against amino acids 371-560 of IRE1α of …
WebDescription When detecting phospho-IRE1 Alpha (Ser-724) using NB100-2323, it is recommended to normalize its band intensity/immunoreactivity with total-IRE1 alpha. NB100-2324 and NB110-59971 can be used for the detection of ... and total IRE1 alpha Antibody [NB100-2324SS] before purchasing 100ug full vials. Product Application Details Web288 rows · IRE1 p Ser724 antibody (NB100-2323) antibody has been validated in …
WebSep 21, 2024 · The primary antibodies used in this study were as follows: XBP1 (Abcam, ab37152), ATF6 (Abcam, ab37149), phospho-IRE1 (Abcam, ab48187), ZIKV envelope protein (BioFront Technologies, BF-1176-56). Statistical analysis All experiments were reproducible and carried out in triplicate. Webphospho ire1 alpha phospho-ire1 alpha p-ire1 alpha Background Unfolded protein response (UPR) signaling is a mechanism used by eukaryotic cells to deal with endoplasmic reticulum/ER stress: an accumulation of unfolded proteins in the ER lumen.
WebThe protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity …
WebDescription. IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. the origin of america police officerWebAnti-phospho IRE-1 (Cat. No. ab48187; 1:2 ,500) antibody was procured from Abcam ( Cambridge, MA, USA). The dilution factors used for Western blotting May 1, 2024 Tae Jin Kim, Do Hyeon Pyun, Myeong Jun Kim, Ji Hoon Jeong, A.M. Abd El-Aty, Tae Woo Jung the origin of antiferroelectricity in pbzro3WebImmunofluorescent analysis of Phospho-IRE1 alpha (Ser724) in Hela cells using a Phospho-IRE1 alpha (Ser724) monoclonal antibody (Product # MA5-33135) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. the origin of anthropologyWebAnti-IRE1 (phospho S724) antibody [1F12] See all IRE1 primary antibodies Description Rabbit monoclonal [1F12] to IRE1 (phospho S724) Host species Rabbit Tested applications Suitable for: ICC, WB more details Species … the origin of ankole kingdomWebTo characterize our anti–phospho-S729 antibody, we treated naive XBP1-proficient and -deficient B cells with LPS for a course of 72 h, immunoprecipitated IRE1 using an anti–mouse IRE1 antibody, and showed that this antibody recognizes the 130-kD phospho-IRE1 protein produced by 48-h and 72-h LPS-stimulated XBP1-deficient B cells . To ... the origin of animalsWebAnti-IRE1α antibody produced in rabbit (Anti-Endoplasmic reticulum to nucleus signaling 1 ); ~ 1mg/mL, affinity isolated antibody, buffered aqueous solution; Suitable for western blot; Yale Center for High Throughput Cell Biology IF-tested antibodies; Anti-IRE1α antibody produced in rabbit has USEN ApplicationsProductsServicesSupport I6785 the origin of arabic languageWebOur Anti-IRE1 (Ser724) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse IRE1 (Ser724) and is antigen affinity purified from pooled serum. It is great for use in WB. Western blot of mouse whole brain lysate showing specific labeling of the ~130 kDa IRE1 protein phosphorylated at ... the origin of a stream